Quantification of biofilm in microtiter plates

overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci

STEPANOVIĆ, SRDJAN
VUKOVIĆ, DRAGANA
HOLA, VERONIKA
DI BONAVENTURA, GIOVANNI
DJUKIĆ, SLOBODANKA
ĆIRKOVIĆ, IVANA
RUZICKA, FILIP

¹Department of Bacteriology, Institute of Microbiology and Immunology, School of Medicine, Belgrade, Serbia, ²Institute for Microbiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic and ³Laboratory of Clinical Microbiology, Aging Research Center Ce.S.I., “G. D'Annunzio” University of Chieti-Pescara, Chieti, Italy

Srdjan Stepanović, Institute of Microbiology and Immunology, School of Medicine, Dr Subotića 1, 11000 Belgrade, Serbia. e-mail [email protected]

The work of SS, DV, SD, and IC was supported by the Ministry of Science, Republic of Serbia (project grant no. 145069B).

Received 8 November 2006.

Accepted 13 February 2007.

APMIS 115(8):p 891-899, August 2007.

The details of all steps involved in the quantification of biofilm formation in microtiter plates are described. The presented protocol incorporates information on assessment of biofilm production by staphylococci, gained both by direct experience as well as by analysis of methods for assaying biofilm production. The obtained results should simplify quantification of biofilm formation in microtiter plates, and make it more reliable and comparable among different laboratories.