BUSCO

assessing genome assembly and annotation completeness with single-copy orthologs

Simão, Felipe A.
Waterhouse, Robert M.
Ioannidis, Panagiotis
Kriventseva, Evgenia V.
Zdobnov, Evgeny M.

Department of Genetic Medicine and Development, University of Geneva Medical School and Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211 Geneva, Switzerland

*To whom correspondence should be addressed.

^†The authors wish it to be known that, in their opinion, the first two authors should be regarded as Joint First Authors.

Associate Editor: John Hancock

Received March 30, 2015

Received in revised form March 30, 2015

Accepted May 21, 2015

Bioinformatics 31(19):p 3210-3212, October 01, 2015. | DOI: 10.1093/bioinformatics/btv351

Motivation: Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50.

Results: We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO.

Availability and implementation: Software implemented in Python and datasets available for download from http://busco.ezlab.org.

Contact:[email protected]

Supplementary information: Supplementary data are available at Bioinformatics online.