Alpha-ketoglutarate modulates the reprogramming of mitochondrial and nuclear DNA methylation during the earliest stages of the bovine preimplantation embryo development†

Ispada, Jessica
de Lima, Camila Bruna
da Fonseca-Junior, Aldcejam Martins
dos Santos, Erika Cristina
Martin, Hélène
Ross, Pablo Juan
Sirard, Marc-Andre
Milazzotto, Marcella Pecora

Laboratory of Embryonic Metabolism and Epigenetics, Centre of Natural and Human Science, Federal University of ABC, Santo Andre, SP, Brazil
Laboratory of Embryonic Metabolism and Epigenetics, Centre of Natural and Human Science, Federal University of ABC, Santo Andre, SP, Brazil
Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle (CRDSI), Département des Sciences Animales, Université Laval, Québec, QC, Canada
Laboratory of Embryonic Metabolism and Epigenetics, Centre of Natural and Human Science, Federal University of ABC, Santo Andre, SP, Brazil
Laboratory of Embryonic Metabolism and Epigenetics, Centre of Natural and Human Science, Federal University of ABC, Santo Andre, SP, Brazil
Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle (CRDSI), Département des Sciences Animales, Université Laval, Québec, QC, Canada
STGenetics, Navasota, TX, United States of America
Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle (CRDSI), Département des Sciences Animales, Université Laval, Québec, QC, Canada
Laboratory of Embryonic Metabolism and Epigenetics, Centre of Natural and Human Science, Federal University of ABC, Santo Andre, SP, Brazil

Correspondence: Laboratory of Embryonic Metabolism and Epigenetics, Centre of Natural and Human Science, Federal University of ABC, Santo Andre, SP, Brazil. E-mail: [email protected]

Grant Support: This work was supported by grant #2019/20982-7, #2018/11668-6, São Paulo Research Foundation (FAPESP), the Natural Sciences and Engineering Research Council - Canada #CG140871, #CG029166 and Coordination of Superior Level Staff Improvement for the student’s scholarships.

Received August 28, 2024

Received in revised form March 11, 2025

Accepted August 15, 2025

Revised August 17, 2025

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/pages/standard-publication-reuse-rights)

Biology of Reproduction 113(3):p 527-540, September 2025. | DOI: 10.1093/biolre/ioaf119