Overproduction of an extracellular polysaccharide possessing high lipid emulsion stabilizing effects by Bacillus sp.

  • Yun, Un-Jung
  • Park, Heui-Dong

  • 1Department of Food Science and Technology, Kyungpook National University, Taegu, 702–701, Korea.

e-mail: [email protected]

Biotechnology Letters 22(8):p 647-650, April 2000. | DOI: 10.1023/A:1005635512860

Abstract

Bacillus sp. CP912, producing an extracellular biopolymer, was isolated from the soil. Maximum accumulation of the biopolymer was 10 g l-1 culture broth with a yield of 88% from glucose consumed. The biopolymer was purified with several precipitation steps using ethanol and cetyl-trimethyl-ammonium bromide. Carbohydrate analyses using various color reactions, infrared spectroscopy, and high performance liquid chromatography revealed that the biopolymer is a homopolysaccharide. The lipid emulsifying capacity of the polysaccharide was 100%, while that of xanthan gum was 94%.

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