SymbolTAT-PDX1 protein induces the differentiation of human fetal liver mesenchymal stem cells into islet beta cells
- Jiang, Hua
- Zhang, Yuan
- Li, Dong-sheng
- Liang, Qing-le
1Institute of Hematology, Medical College of Jinan University, Guangzhou 510632, Guangdong Province, China;
2 Key Laboratory of Embryonic Stem cell Research of Hubei Province, Taihe Hospital, Yunyang Medical College, Shiyan 442000, Hubei Province, China
Jiang Hua☆, Studying for doctorate, Institute of Hematology, Medical College of Jinan University, Guangzhou 510632, Guangdong Province, China; Key Laboratory of Embryonic Stem cell Research of Hubei Province, Taihe Hospital, Yunyang Medical College, Shiyan 442000, Hubei Province, China [email protected]
Correspondence to: Zhang Huan, Doctor, Researcher, Institute of Hematology, Medical College of Jinan University, Guangzhou 510632, Guangdong Province, China [email protected]
Li Dong-sheng, Doctor, Professor, Key Laboratory of Embryonic Stem cell Research of Hubei Province, Taihe Hospital, Yunyang Medical College, Shiyan 442000, Hubei Province, China [email protected]
Supported by: Natural Science Foundation of Hubei Province, No.2007ABA128*
Received: 2008-06-03
Accepted: 2008-10-29
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Abstract
BACKGROUND:
PDX1 is a key factor in dominate the function of isletβ cells, if TAT-PDX1 proteincan be induced to cells and play its role, it will further push the treatment of diabetes.
OBJECTIVE:
To study the capability of differentiation of human fetal liver mesenchymal stem cells (hFLMSCs) intoβ cells by TAT-PDX1 protein in vitro.
DESIGN, TIME AND SETTING:
The in vitro cytology experiment was performed at the Key Laboratory of Embryonic Stem cell Research of Hubei Province, Taihe Hospital, Yunyang Medical College between May 2007 and March 2008.
MATERIALS:
The abortus was from healthy healthy pregnant women with HBsAg (-). The TAT-PDX1 protein was supplied by Tang Dongqi, a professor of University of Florida Health Science Center.
METHODS:
hFLMSCs were isolated from fetal liver by adherent method, the third passages of descended cells were induced with 20 mol/L TAT-PDX1 protein, additionally, DMEM/F12 culture medium contains glucose were added at 4, 6, 8, 10 days, respectively.
MAIN OUTCOME MEASURES:
The morphologic change of cells, the expression ofβ cell specific gene and insulin secretion were detected by RT-PCR, radioimmunoassay and dithizone staining, respectively.
RESULTS:
hFLMSCs can easily separation and amplification in vitro. After induced with TAT-PDX1 protein, the cells turned from spindle to round shape, and gather as islet-like clusters, which showed brownish red color under dithizone staining. The β cell specific gene, such as NeuroD, Nkx2.2, Pax4, Nkx6.1, Pax6 and Isl-1 gene orderly expressed at 4, 6, 8, 10 days after induction. Cumulative release quantity of insulin increased gradually, at 2-4 weeks after induction, the basal and glucose-induced insulin releases significantly increased and maintain a high level.
CONCLUSION:
The TAT-PDX1 protein can induce hFLMSCs differentiate into isletβ cells in vitro.
Jiang H, Zhang H, Li DS, Liang QL. TAT-PDX1 protein induces the differentiation of human fetal liver mesenchymal stem cells into islet beta cells. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2009;13(1):48-52 [http://http://www.crter.cn http://en.zglckf.com]
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