SymbolIdentification of the proteins in the normal and degenerative intervertebral discs by two-dimensional electrophoreses and mass spectrometry
- Lü, Zhi-de
- Zhou, Chu-song
- Jin, An-min
- Liu, Ya-wei
- Zhao, Liang
- Yu, Bo
- Priscilla, Heung
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Department of Orthopaedics, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou 510280, Guangdong Province, China
Lü Zhi-de★, Studying for master's degree, Department of Orthopaedics, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou 510280, Guangdong Province, China [email protected]
Correspondence to: Zhou Chu-song, Chief physician, Associate professor, Department of Orthopaedics, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou 510280, Guangdong Province, China [email protected]
Received: 2008–09–22
Accepted: 2008–12–22
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Abstract
BACKGROUND:
It has made a great progress in the proteomics research of degenerative lumbar disc tissues, however, it is rarely reported that separation and identification of the composition and difference as a whole between normal and degenerative lumbar intervertebral disc.
OBJECTIVE:
To establish a two-dimensional electrophoreses system for identifying the proteins in normal and degenerative intervertebral disc, in addition, to explore the biological mechanism of lumbar disc degeneration.
DESIGN, TIME AND SETTING:
The comparative observation was performed in the Pathological and Physiological Laboratory of Southern Medical University between June 2007 and December 2008.
MATERIALS:
Six normal and six degenerative lumbar intervertebral disc tissues were provided by volunteers, including 9 male and 3 female, which aged between 21–45 years. L3–5 of the lumbar intervertebral disc was harvested.
METHODS:
Proteins were extracted from normal and degenerative lumbar intervertebral disc, the aggrecan was removed by cesium chloride gradient centrifugation, and proteins were separated by two-dimensional electrophoreses and identified by matrix assisted laser adsorption/ionization time-of flight mass spectrometry (MALDI-TOF-MS).
MAIN OUTCOME MEASURES:
IPG two-dimensional gel electrophoresis of the normal and degenerative lumbar intervertebral disc.
RESULTS:
The two-dimensional electrophoresis results showed that there was an obvious difference between the normal and degenerative lumbar intervertebral disc. A total of 6 differential proteins with statistical significance, were identified by MALDI-TOF-MS between the 16 differential protein spots.
CONCLUSION:
The different proteins in normal and degenerative lumbar intervertebral disc can be separated effectively by two-dimensional electrophoresis, and identified by using mass spectrometry.
Lü ZD, Zhou CS, Jin AM, Liu YW, Zhao L, Yu Bo. Priscilla Heung. Identification of the proteins in the normal and degenerative intervertebral discs by two-dimensional electrophoreses and mass spectrometry.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2009;13(7):1271–1274(China) [http://www.crter.cnhttp://en.zglckf.com]
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