SymbolEffects of stilbene glucoside on hypoxia-reoxygenation injury of human umbilical vein endothelial cells
- Liu, Li-ping
- Yang, Yao-fang
- He, Ming
- Li, Wei-dong
- Shao, Li-jian
- Chen, He-ping
- Li, Qing
- Liu, Dan
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1Department of Pharmacolocy, Nanchang University Medical College, Nanchang 330006, Jiangxi Province, China; 2Jiujiang University Medical College, Jiujiang 332000, Jiangxi Province, China
Liu Li-ping★, Master, Senior Experimentalist, Department of Pharmacolocy, Nanchang University Medical College, Nanchang 330006, Jiangxi Province, China; Jiujiang University Medical College, Jiujiang 332000, Jiangxi Province, China [email protected]
Correspondence to: He Ming, Doctor, Professor, Department of Pharmacolocy, Nanchang University Medical College, Nanchang 330006, Jiangxi Province, China [email protected]
Yang Yao-fang, Master's supervisor, Professor, Jiujiang University Medical College, Jiujiang 332000, Jiangxi Province, China [email protected]
Received: 2008–05–31
Accepted: 2008–11–20
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Abstract
BACKGROUND:
Previous studies exhibit that stilbene glucoside, which is the main active component of poygonum multiflorum root, has effects of reducing blood lipid, anti-atherosclerosis and protection of cardiovascular. Active oxygen can result in lipid over-oxidation injuries, lead to the changes of cellular transport function or enzyme properties, accordingly, lose the regulating function on ion channels.
OBJECTIVE:
To investigate the effect of stilbene glucoside on hypoxia-reoxygenation injury of human umbilical vein endothelial cells (HUVECs).
DESIGN, TIME AND SETTING:
The randomized controlled experiment based on HUVECs was performed at the Experiment Center of Molecular Biology from April to October 2006.
MATERIALS:
Human umbilical vein endothelial cells line.
METHODS:
The HUVECs were injured by hypoxia reoxygenation. The intervention experiment was divided into the control, hypoxia-reoxygenation and stilbene glucoside groups, with each group were repeated for 6 times. In the hypoxia-reoxygenation group, HUVECs were hypoxia for 3 hours, following reoxygenation 2 hours. The stilbene glucoside group was assigned into pretreatment and treatment sub-groups, with 10 ng/L, 5 ng/L and 2.5 ng/L co-cultured with HUVECs prior to or after hypoxia-reoxygenation injury, respectively. HUVECs exposed to normoxia were served as the control group.
MAIN OUTCOME MEASURES:
The cell viability was detected by MTT method. Total antioxidant capacity (T-AOC), superoxide dismutase (SOD) and the contents of malondialdehyde (MDA), as well as nitrogen monoxidum (NO) was measured by UV-1206 ultraviolet absorption spectrometer.
RESULTS:
Stilbene glucoside could increase the cell viability, decrease lactate dehydrogenase activety and MDA concentration; lift the T-AOC, SOD activity, as well as NO concentration in a dose-dependent manner (P < 0.01). The effect in the treatment was significant than the pretreatment group.
CONCLUSION:
Stilbene glucoside protects HUVECs against hypoxia-reoxygenation injury, which has superior therapeutical effect to that of prevention.
Liu LP, Yang YF, He M, Li WD, Shao LJ, Chen HP, Li Q, Liu D. Effects of stilbene glucoside on hypoxia-reoxygenation injury of human umbilical vein endothelial cells.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2009;13(7): 1320–1323(China) [http://www.crter.cnhttp://en.zglckf.com]
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