Biocompatibility of the nano-hydroxyapatite/chitosan composite and rabbit bone marrow stem cells
- Yong, Xu
- Li-xin, Zhu
- Jing, Tian
- Bo, Yu
- Qing-ling, Feng
- Zhi, Huang
Abstract
BACKGROUND:
The composite of nano-hydroxyapatite/chitosan (nHA/CS), which prepared by Professor Feng Qin-ling, has good biocompatibility and osteoconductive ability. However, the biocompatibility of the composite is poorly understood.
OBJECTIVE:
To evaluate the biocompatibility between nHA/CS composite and rabbit bone marrow stem cell (BMECs) in vitro.
DESIGN, TIME AND SETTING:
An in vitro observation was performed at the Central Laboratory of Zhujiang Hospital, Southern Medical University from August to November2008.
MATERIALS:
The nHA/CS composite with porosity of 90%, pore size 50-200 μm, was provided by Department of Materials, Qinghua University. BMECs were obtained from 2-week-old healthy rabbit's femur and tibia bone marrow by density gradient centrifugation.
METHODS:
The nHA/CS composite was co-cultured with the third passage of BMECs at a culture plate. The culture BMECs group was served as the control group.
MAIN OUTCOME MEASURES:
The growth of cells was observed by contrast phase microscope and scanning electron microscope. Cell attachment on nHA/Cs was count at 1, 2, and 4 hours after seeding. Proliferation of cell was analyzed by CCK-8 assay. In addition, the cell cycle was detected by flow cytometry.
RESULTS:
The BMSCs grow well on the surface of nHA/CS. The attachment rate in the nHA/CS group was lower than that of the control group at 1 hour (P < 0.05); however, there was no significant difference after 2 and 4 hours (P > 0.05). After seeding, the proliferation of cell had no changes (P > 005). Normal diploid cells could be found in both groups, which indicated that nHA/CS had no influence to cell cycle.
CONCLUSION:
nHA/CS has an excellent biocompatibility with BMECs in vitro.
Figure
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