SymbolPrecaution efficacy of poly-d,l lactic acid/neurotrophic growth factor compound conduit on denervated muscle atrophy

BACKGROUND:

Previous research has shown that poly-d,l lactic acid (PDLLA)/neurotrophic growth factor (NGF) compound conduits could promote regeneration of ischemic peripheral nerves, while the conduits gradually degraded in vivo.

OBJECTIVE:

To determine whether the PDLLA/NGF compound conduits could effectively prevent denervated muscle atrophy and promote functional recovery of target muscle at earlier period after promoting neural regeneration.

DESIGN, TIME AND SETTING:

A randomized and controlled animal experiment was performed at the Department of Anatomy, Southern Medical University from April 2006 to March 2008.

MATERIALS:

The pure PDLLA conduits and the PDLLA/NGF compound conduits were made with solvent-evolution method. Each conduit was with an internal diameter of 1.5 mm, a wall thickness of approximately 0.3 mm and a length of 14 mm. Each compound conduit contained 400U NGF.

METHODS:

A total of 32 SD rats were randomly divided into two groups on average: PDLLA group and PDLLA/NGF compound conduits group. The middle part of right sciatic nerve was excised and bridged with corresponding conduit. 10 mm nerve defect was retained in lumina of conduit.

MAIN OUTCOME MEASURES:

The precaution effect was evaluated according to the gross observation, triceps weight and histological indexes at day 5, 10, 15, and 20 postoperatively. Myocyte diameter and section area of triceps muscle of calf were quantitatively analyzed and contrasted between two groups at each stage.

RESULTS:

The right hindlimbs limped and showed thinningz in all rats after the operation. The recovery rate of triceps weight decreased gradually following the observation period lasted. There were no significant difference between PDLLA group and PDLLA/NGF compound conduits group at every stage (P > 0.05). Normal muscle fiber of triceps appeared uniform shape and regulation alignment. But when it came to the 5th day after operation, the muscle fiber showed smaller diameter. General atrophy was apparent on the 20th day, various appearances, hclewglnouo vaccine sizes and fibroplasias around the muscle bundle. Image analysis showed that the myocyte diameter and section area of triceps reduced postoperatively, without significant difference between the two groups (P > 0.05).

CONCLUSION:

The PDLLA/NGF compound conduit has no significant depressant effect on denervated muscle atrophy at earlier period after bridging the sciatic nerve defect of rat (within 20 days after injury).

Dong HR, Xu DC. Precaution efficacy of poly-d,l lactic acid/neurotrophic growth factor compound conduit on denervated muscle atrophy. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2009;13(21): 4017-4021. [http://www.crter.cnhttp://en.zglckf.com]