SymbolMyogenic regulatory factor Myf-5 expression at different periods of denervated skeletal muscle atrophy
- Zhang, Wen-hui
- Liang, Bing-sheng
- Liang, Yong
- Hou, Zhi-ru
Department of Orthopedics, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
Zhang Wen-hui, Master, Department of Orthopedics, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China [email protected]
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Correspondence to: Liang Bing-sheng, Master's supervisor, Doctoral supervisor, Department of Orthopedics, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
Received: 2008-12-05
Accepted: 2009-04-10
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Abstract
BACKGROUND:
Myogenic regulatory factors Myf-5 is an importance gene involved in muscle occurrence process to adjust and control, start and maintain the skeleton muscle cell growth. It has a possible relation with denervated skeletal muscle atrophy. OBJECTIVE: To detect the expression of Myf-5 gene in different skeletal muscle sites and different periods following denervated skeletal muscle atrophy.
DESIGN, TIME AND SETTING:
Randomized control animal experiment was performed in the Shanxi Medical University between March and April in 2008.
MATERIALS:
A total of 24 Sprague-Dudley male rats was selected and divided randomly into four groups, with six in each, i.e. sham operated group (innervations), denervated 2 d group, denervated 7 d group and denervated 28 d group.
METHODS:
Sham operations were made only for the rats in the sham operated group and sciatic nerve were not cut off. Sciatic nerves were cut off more than one centimeter at the mid-level of their right lower limb for the rats in denervated groups. The rats were executed by vertebrae dislocation method at 2, 7, 28 days after denervation. The skeletal muscles of the right lower limb (tibialis anterior, soleus, gastrocnemius and plantar) were dissected and dissociated.
MAIN OUTCOME MEASURES:
Myf-5 mRNA expression was detected by reverse transcription-polymerase chain reaction. Immunohistochemical stain (ABC method) with polyclonal antibody against Myf-5 protein was performed and the gray value was counted.
RESULTS:
Expressions of Myf-5 mRNA in denervated skeletal muscle were up-regulated at 2, 7, 28 days in the early stage of denervation (P < 0.05). The number of Myf-5 antibody positive-stained cell nuclei was the most in satellite cells at 28 days following denervation.
CONCLUSION:
At the early stage of denervated skeletal muscle atrophy in SD rats, expressions of Myf-5 in different skeletal muscles are all up-regulated. Denervation of rat skeletal muscle raises the Myf-5 expression in satellites cells.
Zhang WH, Liang BS, Liang Y, Hou ZR. Myogenic regulatory factor Myf-5 expression at different periods of denervated skeletal muscle atrophy.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2009;13(28): 5515-5518. [http://www.crter.cnhttp://en.zglckf.com]
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