SymbolEffect of neuropeptide Y on bone mineral density after bilateral sciatic and femoral denervation and immobilization

Li, Zhi-hong
Zhou, Zhen-hua
Xie, Ju-ying

¹Department of Physical Education, Hunan City University, Yiyang 413000, Hunan Province, China; ²Department of Rehabilitation, Xiangnan University Affiliated Hospital, chenzhou 423000, Hunan Province, China
Li Zhi-hong, Master, Associate professor, Department of Physical Education, Hunan City University, Yiyang 413000, Hunan Province, China [email protected]

Supported by: Projects Sponsored by Hunan Provincial Education Ministry, No. 04C170*

Received: 2009-05-16

Accepted: 2009-06-22

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Journal of Clinical Rehabilitative Tissue Engineering Research 13(33):p 6428-6432, August 13, 2009.

Abstract

BACKGROUND:

Previous studies have showed that stimulating the sciatic nerve and central nerve injury may alter the content of neuropeptide Y (NPY), which influences the osteoclasts and bone metabolism.

OBJECTIVE:

To observe the effect of NPY on bone mineral density (BMD) after bilateral sciatic denervation and immobilization, and to perform the correlation analysis.

DESIGN, TIME AND SETTING:

Randomized controlled animal experiment was completed in the Affiliated Hospital of Xiangnan University from September to November in 2006.

MATERIALS:

Ninety-six male Sprague Dawley rats, aged 10 weeks, weighing 220-225 g, were used to prepare denervated models.

METHODS:

According to random number table, 96 rats were divided into 3 groups with 32 rats in each group. ①The denervated group: The bilateral sciatic nerve and femoral nerve were cut off in rats, dissected distally 5 mm, and then the incision was sutured. ②Immobilized group: Rats were performed denervation surgery as above procedures, then immobilized with plaster cast. ③In the control group, only sham operation was performed, the nerves were exposed and then sutured.

MAIN OUTCOME MEASURES:

General conditions of the rats during experiment; eight rats were killed at 1, 10, 30, 60 days after the model preparation to determine the content change of NPY and bilateral BMD, as well as correlation between NPY and BMD.

RESULTS:

All 96 rats were included in the final analysis. ①Following denervation, the content of NPY was decreasing at first and then increasing. With the prolong of denervation duration, the content of NPY soared up, exceeding normal levels. ②In the immobilized group, the content of NPY increased at first and bilateral then decreased. With the prolong of immobilization time, the content of NPY was shown to gradually increase and exceeded the normal levels. ③With the altering content of NPY, the BMD in bilateral tibial bone also changed and it decreased obviously at 30 days following denervation and immobilization. The NPY content was highly correlated with the BMD following denervation and immobilization (P < 0.01).

CONCLUSION:

The content of NPY is highly related with BMD in denervated and immobilized groups, so it can be observed as a key factor of osteoporotic formation.

Li ZH, Zhou ZH, Xie JY. Effect of neuropeptide Y on bone mineral density after bilateral sciatic and femoral denervation and immobilization.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2009;13(33): 6428-6432. [http://http://www.crter.cn http://en.zglckf.com]

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