SymbolReparation of tibial defects using adipose-derived stromal cells combined with bovine decalcified bone in rabbits
- Chen, Wei
- Jiang, Yi
Tianjin Third Central Hospital, Tianjin 300170, China
Chen Wei, Physician, Tianjin Third Central Hospital, Tianjin 300170, China [email protected]
Supported by: the Science Foundation of Tianjin Health Bureau, No.07ky15*
Received: 2009-05-27
Accepted: 2009-06-29
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Abstract
BACKGROUND:
Previous studies confirmed that adipose-derived stromal cells have bone activeness after induction. Therefore, it attracts a lot of attention in the context of repair bone damage with adipose-derived stromal cells. However, the reports regarding repairing bone defects in a heavy load area with adipose-derived stromal cells are few.
OBJECTIVE:
To explore the feasibility of adipose-derived stromal cells in repairing loaded bone defects through observing the repair of rabbit tibial bone defects with adipose-derived stromal cells that was cultured to osteoplast and bovine decalcified bone composite.
DESIGN, TIME AND SETTING:
The randomized control animal experiment was performed at the animal laboratory of Tianjin Third Central Hospital from January to September 2008.
MATERIALS:
The demineralized bone was prepared with cancellated bone of metaphysis from newborn calf via treatment of decalcification, defatting, acellular, remove antigen, and deproteinization.
METHODS:
Twelve adult, healthy, New Zealand white rabbits. One animal was randomly selected, 15 mL intra-abdominal adipose tissue was extracted, cultured and in vitro induction to osteoblasts, followed by plantation on demineralized bone scaffold with density of 1×109 /L, and continue to cultivate a week to produce tissue-engineered bone. The 12 rabbits were prepared for bilateral tibial bone defects model. The left lower extremity was implanted demineralized bone served as a control group, and the right lower extremity implanted in bone tissue engineering as the experimental group.
MAIN OUTCOME MEASURES:
X-ray film, CT examination was performed to observe callus formation, and gross observation was used to detect the reparation of defects.
RESULTS:
Gross observation and X-ray showed that the bone defect disappeared and bone had healing in the experimental group at 12 weeks after operation. But bone defect still existed, without fully healing in the control group. Histological observation showed that bone trabecula had formed in the experimental group, but no new bone formation in the control group at 12 weeks after operation. Line measurement of CT value pair t test showed that the experimental group was significantly better than the control group.
CONCLUSION:
Adipose tissue-derived stromal cells have osteogenic activity after induction culture, which are able to repair bone defects combined with bovine decalcified bone.
Chen W, Jiang Y.Reparation of tibial defects using adipose-derived stromal cells combined with bovine decalcified bone in rabbits.Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2009;13(34):6650-6654. [http://www.crter.cnhttp://en.zglckf.com]
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