SymbolEffect of nickel-titanium alloy surface oxidation on L-929-related apoptotic gene bax/bcl-2 mRNA expression
- Feng, Cui-juan
- Wang, Qiang
- Zhang, Xue
- Zhang, Yang
- Qin, Ke
- Lu, Li
1Department of Orthodontics, 2Department of Oral Materials, 3Department of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China
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Feng Cui-juan☆, Studying for doctorate, Lecturer, Department of Orthodontics, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China [email protected]
Correspondence to: Lu Li, Professor, Doctoral supervisor, Department of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China [email protected]
Correspondence to: Zhang Yang, Professor, Doctoral supervisor, Department of Orthodontics, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China [email protected]
Supported by: the Natural Science Foundation of Liaoning Province, No. 20072106*, 20082088*
Received: 2010–01–07
Accepted: 2010–02–22
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Abstract
BACKGROUND:
Nickel-titanium memory alloy released toxic Ni2+ following corrosion, which could seriously influence human body. Inhibitory coating of TiN, TiO2, and HA was made at alloy surface, respectively, while the coating might effectively inhibit release of Ni2+ and improve the biocompatibility.
OBJECTIVE:
To evaluate the effect of nickel-titanium memory alloy following surface oxidation on biocompatibility.
METHODS:
NiTi-SMA was treated with surface oxidation. The sample was then rinsed with 10% HF + 40 % HNO3 + 50% H2O, ultrasonic-cleared in acetone, dried, oxygenized in H2O2, rinsed, dried, sterilized, and lixiviated. L-929 cells were cultured in vitro and assigned into negative control (culture medium), surface oxidation (surface oxidation + NiTi-SMA leaching liquor), and non-treatment groups (NiTi-SMA leaching liquor alone). Apoptotic gene bax/bcl-2 mRNA expressions were measured by PT-PCR.
RESULTS AND CONCLUSION:
bax/bcl-2 mRNA expression in the negative control group was significantly higher than surface oxidation and non-treatment groups, but bax/bcl-2 in the negative control group was significantly lower than surface oxidation and non-treatment groups (P < 0.01). There was no significant difference in bax/bcl-2 mRNA and bax/bcl-2 between surface oxidation and non-treatment groups (P > 0.05). This suggested that the nickel ion releasing from nickel-titanium memory alloy leaching liquor could result in the variation of bax/bcl-2 mRNA expression in L-929, which may be used as an important index to evaluate the biocompatibility of biomaterials.
Feng CJ, Wang Q, Zhang X, Zhang Y, Qin K, Lu L.Effect of nickel-titanium alloy surface oxidation on L-929-related apoptotic gene bax/bcl-2 mRNA expression. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2010;14(12): 2145–2148.
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