SymbolNano-hydroxyapatite/collagen composite co-cultured with bone marrow-derived mesenchymal stem cells for repair of bone defects
- Zhao, Ji-dong
- Qian, Han-guang
- Miao, Zong-ning
- Zhu, Jian-zhong
- Peng, Wei
Department of Reparative and Reconstructive Surgery, Wuxi Third People's Hospital, Wuxi 214041, Jiangsu Province, China
Zhao Ji-dong, Department of Reparative and Reconstructive Surgery, Wuxi Third People's Hospital, Wuxi 214041, Jiangsu Province, China
Zhao JD, Qian HG, Miao ZN, Zhu JZ, Peng W.
Nano-hydroxyapatite/ collagen composite co-cultured with bone marrow-derived mesenchymal stem cells for repair of bone defects. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu 2010;14(42): 7971-7975.
Symbol
no caption available.
Received: 2010-06-10
Accepted: 2010-08-11 (20100330031/YJ)
[http://www.crter.cnhttp://en.zglckf.com]
Symbol
no caption available.
Symbol
no caption available.
Abstract
BACKGROUND:
There have been no effective means for clinical treatment of large regions of bone defects. Nano-hydroxyapatite/collagen (nHAC) composite would provide a new pathway for repair of bone defects owing to its similar structure to natural skeleton and better biocompatibility.
OBJECTIVE:
To investigate the role of nHAC composite co-cultured with bone marrow-derived mesenchymal stem cells (BMSCs) in repair of bone defects.
METHODS:
Following isolation and culture, human BMSCs were co-cultured with nHAC composite. Gross observation, histological analysis, and electron microscope observation were performed to analyze osteogenesis for repair of bone defects in the clinic.
RESULTS AND CONCLUSION:
Human nHAC could greatly proliferate in vitro. X-ray photography revealed that bone defects well healed after implantation of nHAC/BMSCs composite. These findings indicate that BMSCs exhibit osteogenic potential and nHAC is a satisfactory scaffold material for construction of tissue-engineered bone.
Figure
no caption available.