Pulse electric fields effect on invasion and metastasis of Hela cells in human cervical cancer

Zhang, Qin
Xiong, Zheng-ai
Zhou, Wei
Li, Cheng-xiang
Yao, Chen-guo

¹Deparment of Gynaecology and Obsterics, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China; ²Chongqing University, Chongqing 400030, China

Zhang Qin, Studying for master's degree, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China [email protected]

Correspondence to: Xiong Zheng-ai, Doctoral supervisor, Department of Gynaecology and Obsterics, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China [email protected]

Supported by: the National Natural Science Foundation of China, No. 50637020*

Received: 2011-01-16

Accepted: 2011-03-24

Journal of Clinical Rehabilitative Tissue Engineering Research 15(28):p 5245-5248, July 9, 2011.

Abstract

BACKGROUND:

The inhibitory effect of pulse electric fields on invesion and metastasis ability of tumor cells is unclear.

OBJECTIVE:

To explore the impact of different intensity pulse electric fields on the human cervical carcinoma Hela cell invasion and metastasis and its possible mechanism.

METHODS:

The electric fields with intensity of 0, 500, 1 000 and 1 500 V/cm were performed on the human cervical carcinoma Hela cell. The clone capacity was measured with flat clone formation experiment; the invasion and immigration capacity was evaluated with transwell cave method;the expression level of mRNAof MMP-2 and RhoC was detected by RT-PCR method.

RESULTS AND CONCLUSION:

After being treated with pulsed electric fields, the clone capacity of Hela cells was inhibited. The invasion and immigration capacity of Hela cells were also inhibited. The expression level of mRNA of matrix metalloproteinase 2 (MMP-2) and RhoC were decreased. The pulse electric fields could affect the invasion and metastasis capacity of the human cervical cancer Hela cells. It is probably one of its molecular mechanisms to inhibit the expression of MMP-2 and RhoC.

Zhang Q, Xiong ZA, Zhou W, Li CX, Yao CG. Pulse electric fields effect on invasion and metastasis of Hela cells in human cervical cancer. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2011;15(28): 5245-5248. [http://www.crter.cnhttp://en.zglckf.com]