SymbolDetection of interleukin 2, interleukin 10, transforming growth factor β and CD4⁺CD25⁺ regulatory T cells in peripheral blood of patients with acute lymphoblastic leukemia

BACKGROUND:

At present, the cause of acute lymphoblastic leukemia (ALL) has not yet entirely understood, CD4⁺CD25⁺ regulatory T cells (Treg) may have certain regulatory effect on immune response in pathogenic process of ALL.

OBJECTIVE:

To study the levels of interleukin 2 (IL-2), interleukin 10 (IL-10), transforming growth factor (TGF-β) of CD4⁺CD25⁺ regulatory T cells (Treg) in peripheral blood of patients with ALL.

METHODS:

The peripheral blood mononuclear cells (PBMC) were isolated with density gradient centrifugation from 22 B cell series ALL (B-ALL) patients, 13 T cell series ALL (T-ALL) patients and 18 normal people. Immunomagnetic bead separation was used to separate CD4⁺CD25⁺Treg cells, and the levels of IL-2, IL-10 and TGF-β in the cells culture supernatant were detected by using enzyme-linked immunosorbent assay (ELISA).

RESULTS AND CONCLUSION:

The levels of IL-10 and TGF-β in the cells culture supernatant of B-ALL patients and T-ALL patients were significantly higher than those in normal people, while the level of IL-2 was significantly lower than that in normal people (P< 0.05). There was no significant difference between B-ALL and T-ALL patients in the IL-2, IL-10 and TGF-β levels of the cells culture supernatant (P > 0.05). CD4⁺CD25⁺Treg cells might interfere the immunologic reaction of antitumor by increasing the IL-10 and TGF-β levels and decreasing the IL-2 level.

Wu CP, Qin X, Wu CY, Zhu H, Zhou HY. Detection of interleukin 2, interleukin 10, transforming growth factor β and CD4⁺CD25⁺ regulatory T cells in peripheral blood of patients with acute lymphoblastic leukemia.Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(14): 2648-2651. [http://www.crter.cnhttp://en.zglckf.com]