SymbolSilk fibroin/hydroxyapatite combined with bone marrow mesenchymal stem cells for construction of tissue engineered cartilage
- Yang, Yao
- Xu, Wei-yuan
- Zhang, Ya
- Zhang, Xing-xiang
- Liu, Zong-bao
- Qian, Hui
- Huang, Jian-ping
- Cui, Zhi-hao
1School of Clinical Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China;
2Department of Orthopaedics, First People's Hospital of Zhangjiagang, Zhangjiagang 215600, Jiangsu Province, China;
3Children's Hospital Affiliated to Soochow University, Suzhou 215003, Jiangsu Province, China
Yang Yao, Studying for master's degree, School of Clinical Medicine, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China [email protected]
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Correspondence to: Xu Wei-yuan, Doctor, Professor, Chief physician, Department of Orthopaedics, First People's Hospital of Zhangjiagang, Zhangjiagang 215600, Jiangsu Province, China [email protected]
Received: 2011-01-17 Accepted: 2011-03-18
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Abstract
BACKGROUND:
With the emergence of tissue engineering, the possibility of repairing the cartilage injury is greatly increased, but single scaffold materials fail to meet ideal scaffold, and have some limitations.
OBJECTIVE:
To explore the feasibility of silk fibroin/hydroxyapatite combined with bone marrow mesenchymal stem cells (BMSCs) for construction of tissue engineered cartilage
METHODS:
BMSCs were isolated, cultured and induced into chondrocytes, which were cocultured with silk fibrion/hydroxyapatite to construct full-thickness articular cartilage defects in the platform of knee joints tibial plateau. A total of 45 rabbits with unilateral knee full thickness cartilage defects model were randomly divided into 3 groups. The compound group was implanted with cell-silk fibrion/hydroxyapatite compound; material group was only implanted with silk fibrion/hydroxyapatite; implantation was not given in the control group. The reparation condition of cartilage defects was observed by CT examination and histological examination at 8, 12 weeks after implantation.
RESULTS AND CONCLUSION:
In the compound group, the articular surface was not flat, the joint space was increased, formed new chondrocyte-like cells and extracellular matrix was extremely rich after 8 weeks. In the material group, articular surface was collapsed and a small amount of chondrocytes were proliferated. After 12 weeks, in the compound group, the articular surface was flat, the joint space was normal; a large number of chondrocytes were emerged, and the color of repaired tissues was closed to surrounding cartilage and scaffolds were completely degraded. In the material group, the articular surface was not flat and cartilage cells did not completely filled, some scaffolds were degraded. The defects were not repaired in the control group. It is indicated that the method of repairing the full-thickness hyaline cartilage defects using BMSCs combined with silk fibroin/hydroxyapatite is feasible, and the silk fibroin/hydroxyapatite can be used as scaffold materials in articular cartilage tissue engineering due to a good biocompatibility.
Yang Y, Xu WY, Zhang Y, Zhang XX, Liu ZB, Qian H, Huang JP, Cui ZH.Silk fibroin/hydroxyapatite combined with bone marrow mesenchymal stem cells for construction of tissue engineered cartilage. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2011;15(29): 5339-5342. [http://www.crter.cnhttp://en.zglckf.com]
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