Effect of laser drilling before vitrified cryopreservation on transplantation of frozen-thawed blastocyts

Fang, Jian-ye
Yang, Jing
Yin, Tai-lang
Cheng, Yan
Zhao, Qing-hong
Zou, Yu-jie
Huang, Wei

Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China
Fang Jian-ye⋆, Studying for master's degree, Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China

Correspondence to: Yang Jing, Doctor, Professor, Doctoral supervisor, Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China [email protected]

Received: 2011-03-29

Accepted: 2011-05-18

Journal of Clinical Rehabilitative Tissue Engineering Research 15(31):p 5731-5734, July 30, 2011.

Abstract

BACKGROUND:

A series of studies have demonstrated that artificially reduced blastocoelic cavity can greatly increase the vitrified frozen-thawed effects of blastocytes, but different physical methods and materials used during the process of reduction can lead to mechanical injury to blastocytes.

OBJECTIVE:

To investigate the effects of laser drilling before vitrified cryopreservation of transplantation of frozen-thawed blastocyts.

METHODS:

Blastocytes from three dysgenesia patients who averaged 30 years old were subjected to laser drilling before vitrified cryopreservation to reduce the blastocoelic cavity for 1 year on average. Before transplantation, these blastocytes were thawed.

RESULTS AND CONCLUSION:

Two patients acquired satisfactory pregnancy and one patient did not get pregnancy. The results showed that laser drilling before vitrified cryopreservation reduces blastocoelic cavity and transplantation of frozen-thawed blastocytes is safe and effective.