Symbol.Subcutaneous implantation of bone marrow mesenchymal stem cells-polyglycolic acid scaffold complex to construct small diameter tissue-engineered blood vessels**☆
- Gao, Guo-liang
- Song, Jian-fei
- Wang, Hai-yong
- Zheng, Min
- Wang, Wei
- Jiang, Yi-yao
- Du, Zhen-zong
Department of Cardiothoracic Surgery, Affiliated Hospital of Guilin Medical University, Guilin 541001, Guangxi Zhuang Autonomous Region, China
Gao Guo-liang☆, Studying for master's degree, Department of Cardiothoracic Surgery, Affiliated Hospital of Guilin Medical University, Guilin 541001, Guangxi Zhuang Autonomous Region, China [email protected]
Gao GL, Song JF, Wang HY, Zheng M, Wang W, Jiang YY, DuZZ.
Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2011;15(51): 9544-9548.
Correspondence to: Du Zhen-zong, Doctor, Associate professor, Associate chief physician, Master's supervisor, Department of Cardiothoracic Surgery, Affiliated Hospital of Guilin Medical University, Guilin 541001, Guangxi Zhuang Autonomous Region, China [email protected]
Supported by: the Healthy Department of Guangxi Zhuang Autonomous Region, No. Z2007199*; Z2008290*
Received: 2011-04-20 Accepted: 2011-06-29 (20110420016/M)
Subcutaneous implantation of bone marrow mesenchymal stem cells-polyglycolic acid scaffold complex to construct small diameter tissueengineered blood vessels.
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Abstract
BACKGROUND:
Our former studies have shown that bone marrow mesenchymal stem cells (BMMSCs) can be induced differentiation to vascular smooth muscle-like cells (VSMLCs) and vascular endothelium-like cells (VELCs), which are compatible with collagen-embedded polyglycolic acid scaffolds.
OBJECTIVE:
To investigate the possibility of constructing small diameter tissue-engineered blood vessels via subcutaneous implantation.
METHODS:
The cells-scaffold complex was produced by separately seeding VSMLCs and VELCs derived from BMMSCs on polyglycolic acid collagen scaffolds. The two layers were separated by ECMgel. The cells-scaffold complex was subcutaneous implanted into small diameter tissue-engineered blood vessels.
RESULTS AND CONCLUSION:
Histological analysis of the small diameter tissue-engineered blood vessel walls revealed a typical artery structure, which was similar to natural vessels. The tissue-engineered blood vessels were not broken down under a force of 26.6 kPa. Eight weeks after implantation, the Brdu-labeled seed cells were found in the three layers of the vessel walls. The results revealed that the subcutaneous tissue was a good bioreactor to construct small diameter tissue-engineered blood vessels.
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