SymbolCytotoxicity of dehydrated ostrich acellular corneal stroma as a carrier material
- Liu, Xian-ning
- Zhu, Xiu-ping
- Wu, Jie
- Wang, Li-fang
- Yin, Yong
(1Shaanxi Institute of Ophthalmology, Xi'an 710002, Shaanxi Province, China; 2Shaanxi Institute for Food and Drug Control, Xi'an 710002, Shaanxi Province, China)
Liu Xian-ning, Researcher, Shaanxi Institute of Ophthalmology, Xi'an 710002, Shaanxi Province, China [email protected]
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Supported by: Innovation Co-ordinator Project of Shaanxi Province, No. 2012KTCQ03–11*
Received: 2013–01–03
Accepted: 2013–01–23
Abstract
BACKGROUND:
Previous studies from Shaanxi Institute of Ophthalmology have shown that ostrich cornea has the advantages to be developed into the alternatives of human corneal material.
OBJECTIVE:
To determine the potential toxic effects of ostrich corneal stromal scaffold on cells.
METHODS:
Cell culture methods were used to culture L-929 cells in the extracts of ostrich acellular corneal stroma which was dried and dehydrated. 3-(4,5)-Dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide assay was used to evaluate the growth and proliferation of cells after cultured for 1, 2 and 3 days.
RESULTS AND CONCLUSION:
After the cells were cultured in the extracts of ostrich acellular corneal stroma subjected to dryness and dehydration for 1, 3 and 5 days, and the toxicity level of cultured cells was graded as level 1. The cytotoxicity test was conducted according to the “National Standard of the People's Republic of China GB/T16886.5–2003”. After cultured in the extracts of ostrich acellular corneal stroma, a small number of cells were round in shape and loosely adherent without intracytoplasmic granules, and cell lysis could be observed occasionally. The results of 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide assay showed that the ostrich acellular corneal stromal scaffold which was dried and dehydrated had level 1 of cytotoxicity and could be considered as a qualified material.
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