Molecular cloning of the cDNAs encoding the feline B-lymphocyte activation antigen B7-1 (CD80) and B7-2 (CD86) homologues which interact with human CTLA4-Ig

Nishimura, Y.
Shimojima, M.
Miyazawa, T.
Sato, E.
Nakamura, K.
Izumiya, Y.
Ikeda, Y.
Mikami, T.
Takahashi, E.

* Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan, † The Research Center for Protozoan Molecular Immunology, Obihiro University, Obihiro, Japan

Received 28 February 2000; revised 26 April 2000; accepted 27 April 2000

Present addresses: ‡Wohl Virion Centre and §Department of Immunology, Windeyer Institute for Medical Science, University College London, 46 Cleveland Street, London W1P 6DB, UK.

The nucleotide sequence data reported in this paper will appear in the DDBJ/EMBL/GenBank nucleotide sequence databases with the accession numbers AB030651 (feline CD80) and AB030652 (feline CD86).

Correspondence: Professor Eiji Takahashi, Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

European Journal of Immunogenetics 27(5/6):p 427-430, October-December 2000.

Summary

We cloned the cDNAs encoding the feline homologues of B-lymphocyte activation antigens B7-1 (CD80) and B7-2 (CD86). We expressed recombinant feline CD80 and CD86 molecules by the baculovirus expression system, and demonstrated their binding ability to human CTLA4-murine immunoglobulin fusion protein.

The B-cell activation antigens B7-1 (CD80) and B7-2 (CD86) are homologous molecules expressed on the antigen presenting cells, interact with the T-cell antigen CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA4), and play important roles in regulation of the immune system . Recently, we cloned the cDNAs encoding the feline CD28 (fCD28) and CTLA4 (fCTLA4) homologues which conserved the hexapeptide motif (MYPPPY) known as the important region for binding of CD28 to CD80 in humans . However, there was no information about feline CD80 (fCD80) and CD86 (fCD86) molecules, and their function and role in feline immune system have not been discussed yet. In this study, we cloned and sequenced the cDNAs encoding the fCD80 and fCD86 homologues which interact with human CTLA4-murine immunoglobulin (Ig) fusion protein.