**Optimized medium culture forAcidobacteriasubdivision 1 strains**

Campanharo, João Carlos
Kielak, Anna Maria
Castellane, Tereza Cristina Luque
Kuramae, Eiko Eurya
de Macedo Lemos, Eliana Gertrudes
Mongkolsuk, Skorn

¹Departamento de Tecnologia, UNESP—Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Rod. Prof. Paulo Donato Castellane km 5, CEP 14884–900 Jaboticabal, SP, Brazil and ²Netherlands Institute of Ecology (NIOO-KNAW), Department of Microbial Ecology, PO Box 50, 6700 AB, Wageningen, the Netherlands

^*Corresponding author: Departamento de Tecnologia, UNESP—Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Rod. Prof. Paulo Donato Castellane km 5, CEP 14884–900 Jaboticabal, SP, Brazil. Tel: +55 16 32092675 ext.217; Fax: +55 16 32092675; E-mail: [email protected]

Accepted: October 25, 2016; Received: June 04, 2016; previously published online November 02, 2016

One sentence summary: New medium formulation named PSYL 5 includes sucrose, as a carbon source, and other compounds (such as KH₂PO₄ and MgSO₄.7H₂O) for members of Acidobacteria subdivision 1.

FEMS Microbiology Letters (FEMSLE) 363(21):p fnw245, November 1, 2016. | DOI: 10.1093/femsle/fnw245

ABSTRACT

Members of subdivision 1 of the phylum Acidobacteria were grown at different pH values in a new medium formulation named PSYL 5, which includes sucrose as a carbon source and other compounds (such as KH₂PO₄ and MgSO₄.7H₂O). Growth rate was nearly constant at pH 5.0 and declined at pH 3–4 and 6–7. However, it was found that effects involving good carbon/nitrogen ratios and pH on the growth of the members of Acidobacteria subdivision 1 were significant, and the strongest effect of these conditions was at pH 5.0. In addition, incubation time of 48, 72, 96 and 120 h was shorter than that described previously for members of Acidobacteria subdivision 1 on solid laboratory media.