Spectrophotometric Methods for the Determination of Nelfinavir Mesylate1

  • Rao, Seshagiri V. M. Mohan
  • Reddy, Tadi R. S.
  • Rao, Isukapatla N.
  • Sastry, Chilukuri S. P.

  • *Department of Organic Chemistry, Foods, Drugs and Water, School of Chemistry, Andhra University, Visakhapatnam-530 003, India

    **Department of Physical and Nuclear Chemistry & Chemical Oceanography, School of Chemistry, Andhra University, Visakhapatnam-530 003, India

***Address for correspondence: Prof. C. S. P. Sastry, D. N. O. 9-36-4, Opp. N. C. C. Office, Andhra Bank Road, Pithapuram Colony, Visakhapatham-530 003, India; e-mail: [email protected]

Journal of Analytical Chemistry 59(6):p 552-556, June 2004.

Three visual spectrophotometric methods are described for the assaying of nelfinavir mesylate (NEL) in either bulk form or dosage forms. Methods A-C are based on the oxidation of NEL with an excess of oxidant (N-bromosuccinimide (NBS) in methods A&B or chloramine-T (CAT) in method C) in acidic medium. The unreacted oxidant is then estimated colorimetrically using an oxidisable dye (Celistine blue (CB) in method A or Gallocyanine (GC) in method C) or p-N-methyl aminophenol sulphate (PMAP)-sulphanilamide (SA) reagent (in method B). These methods obey Beer's law and give reproducible results. Recoveries range from 99.3–100.5%.

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