Same Serial Section Correlative Light and Energy-filtered Transmission Electron Microscopy

Ren, Ying
Kruhlak, Michael J.
Bazett-Jones, David P.

¹Programme in Cell Biology, The Hospital for Sick Children, Toronto, Ontario, Canada

Correspondence to: David P. Bazett-Jones, Programme in Cell Biology, The Hospital for Sick Children, 555 University Avenue, Toronto, ONT M5G 1×8, Canada. E-mail: [email protected]

¹Present address: Experimental Immunology Branch, National Institutes of Health, Bethesda, MD.

Accepted December 6, 2002

Received July 31, 2002

Journal of Histochemistry & Cytochemistry 51(5):p 605-612, May 2003. | DOI: 10.1177/002215540305100506

Correlative imaging of a specific cell with both the light microscope and the electron microscope has proved to be a difficult task, requiring enormous amounts of patience and technical skill. We describe a technique with a high rate of success, which can be used to identify a particular cell in the light microscope and then to embed and thin-section it for electron microscopy. The technique also includes a method to obtain many uninterrupted, thin serial sections for imaging by conventional or energy-filtered transmission electron microscopy, to obtain images for 3D analysis of detail at the suborganelle level.