Immobilization of the cell-adhesive peptide Arg–Gly–Asp–Cys (RGDC) on titanium surfaces by covalent chemical attachment

Xiao, S. J.
Textor, M.
Spencer, N. D.
Wieland, M.
Keller, B.
Sigrist, H.

¹Laboratory for Surface Science and Technology, Department of Materials, Swiss Federal Institute of Technology, CH-8092 Zurich Switzerland.
²Swiss Federal Laboratories for Materials Testing and Research, Uberlandstrasse 129, CH-8600 Dubendorf Switzerland.
³CSEM, Centre Suisse d'Electronique et de Microtechnique SA, Jaquet-Droz 1, CH-2007 Neuchatel Switzerland.

^*Author to whom all correspondence should be addressed.

Journal of Materials Science: Materials in Medicine 8(12):p 867-872, 1997.

Surface modification of acid-pretreated titanium with 3-aminopropyltriethoxylsilane (APTES) in dry toluene resulted in covalently bonded siloxane films with surface coverage that was relatively controllable by regulating the reaction conditions. A hetero-bifunctional cross-linker, N-succinimidyl-3-maleimidopropionate (SMP), reacted with the terminal amino groups, forming the exposed maleimide groups. Finally, a model cell-binding peptide, Arg–Gly–Asp–Cys (RGDC), was immobilized on the surface through covalent addition of the cysteine thiol groups to the maleimide groups. X-ray photoelectron spectroscopy, radiolabelling techniques, and ellipsometry were used to quantify and characterize the modified surfaces.