Concordance Study Between the AmpFℓSTR® MiniFiler™ PCR Amplification Kit and Conventional STR Typing Kits

  • Hill, Carolyn R. M.S.
  • Kline, Margaret C. M.S.
  • Mulero, Julio J. Ph.D.
  • Lagacé, Robert E. B.A.
  • Chang, Chien-Wei Ph.D.
  • Hennessy, Lori K. Ph.D.
  • Butler, John M. Ph.D.

  • 1National Institute of Standards and Technology, Biochemical Science Division, 100 Bureau Drive, Mail Stop 8311, Gaithersburg, MD 20899.

    2Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404.

Additional information and reprint requests: John M. Butler, Ph.D. Research Chemist National Institute of Standards and Technology Biochemical Science Division 100 Bureau Drive, Mail Stop 8311 Gaithersburg, MD 20899-8311 E-mail: [email protected]

Received 2 Dec. 2006; and in revised form 18 Mar. 2007; accepted 18 Mar. 2007; published 4 June 2007.

Journal of Forensic Sciences 52(4):p 870-873, July 2007.

The AmpFℓSTR® MiniFiler™ polymerase chain reaction amplification kit developed by Applied Biosystems enables size reduction on eight of the larger STR loci amplified in the Identifiler® kit, which will aid recovery of information from highly degraded DNA samples. The MiniFiler™ Kit amplifies CSF1PO, FGA, D2S1338, D7S820, D13S317, D16S539, D18S51, and D21S11 as well as the sex-typing locus amelogenin. A total of 1308 samples were evaluated with both the MiniFiler™ and Identifiler® STR kits: 449 African American, 445 Caucasian, 207 Hispanic, and 207 Asian individuals. Full concordance between Identifiler and MiniFiler Kits was observed in 99.7% (10,437 out of 10,464) STR allele calls compared. The 27 differences seen are listed in Table 1 and encompass the loci D13S317 (n = 14) and D16S539 (n = 10) as well as D18S51 (n = 1), D7S820 (n = 1), and CSF1PO (n = 1). Genotyping discrepancies between the Identifiler and MiniFiler kits were confirmed by reamplification of the samples and further testing using the PowerPlex® 16 kit in many cases. DNA sequence analysis was also performed in order to understand the nature of the genetic variations causing the allele dropout or apparent repeat unit shift.

Copyright © 2007 Blackwell Publishing Ltd.