Antioxidant activity and constituents of extracts from the root ofGarcinia multiflora

  • Wu, Jyh-Horng
  • Tung, Yu-Tang
  • Chyu, Chiou-Fung
  • Chien, Shih-Chang
  • Wang, Sheng-Yang
  • Chang, Shang-Tzen
  • Kuo, Yueh-Hsiung

  • 1Department of Forestry, National Chung-Hsing University, Taichung, 402 Taiwan

    2School of Forestry and Resource Conservation, National Taiwan University, Taipei, 106 Taiwan

    3Department of Chemistry, National Taiwan University, Taipei, 106 Taiwan

    4Department of Chemistry, National Taiwan University, Taipei, 106 Taiwan

    5Agricultural Biotechnology Research Center, Academia Sinica, Taipei, 115 Taiwan

    6Tsuzuki Institute for Traditional Medicine, College of Pharmacy, China Medical University, Taichung, 404 Taiwan

e-mail: [email protected]

Received: 15 October 2007/Accepted: 22 February 2008/Published online: 26 June 2008

Journal of Wood Science 54(5):p 383-389, October 2008. | DOI: 10.1007/s10086-008-0961-9

Abstract

The methanolic extract of the root of Garcinia multiflora and its derived soluble fractions, namely those soluble in ethyl acetate (EtOAc), n-butanol, and water, were screened for their antioxidant activities. Among them, the EtOAc-soluble fraction exhibited the highest scavenging activity against the 1,1-dipheny1,2-picrylhydrazyl radical, the highest superoxide radical scavenging activity, and the strongest reducing power. In addition, according to the bioactivity-guided isolation, 26 specific phytocompounds, including 3 aromatics, 3 benzophenones, 3 flavonoids, 3 isocoumarins, 1 phloroglucinol, 6 steroids, and 7 xanthones, were isolated from the EtOAc-soluble fraction and identified. Of these, 2,4,3′,4′-tetrahydroxy-6-methoxybenzophenone and 1,3,6,7-tetrahydroxyxanthone were found to be the major bioactive constituents, present in the crude extract in concentrations of 3.9 and 15.6 mg/g, respectively. These two compounds had similar antioxidant activities to (+)-catechin, a well-known antioxidant.

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