PURIFICATION OF A HUMAN PROSTATE SPECIFIC ANTIGEN1

  • WANG, M. C.
  • VALENZUELA, L. A.
  • MURPHY, G. P.
  • CHU, T. M.

1 This work was supported in part by USPHS Research Grant No. CA-15437, awarded by the National Cancer Institute, DHEW.

2 Send requests for reprints to Dr. T. M. Chu, Roswell Park Memorial Institute, Buffalo, New York 14263.

Submitted for publication March 26, 1979; accepted June 7, 1979.

Journal of Urology 167(3):p 1226-1230, March 2002.

Rabbit antiserum raised against the crude extract of normal human prostatic tissue contained antibodies to a prostatic tissue-specific antigen as shown by immunoprecipitation techniques. Using this antiserum a prostate antigen was detected in normal, benign hypertrophic, and malignant prostatic tissues, but not in other human tissues. The prostate antigen was purified to homogeneity from prostatic tissues and showed a single protein band on analytical polyacrylamide gel electrophoresis and isoelectric focusing. This report thus presents the first demonstration of the purification of a prostate-specific antigen that does not represent prostatic acid phosphatase.

Department of Diagnostic Immunology Research and Biochemistry, Roswell Park Memorial Institute, Buffalo, New York (Reprinted with permission from Invest. Urol, 17: 159–163, 1979)

Copyright © 2002 by the American Urological Association, Inc.