Fast chromatin immunoprecipitation assay

Nelson, Joel D.
Denisenko, Oleg
Sova, Pavel
Bomsztyk, Karol

¹Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98109, USA
²UW Medicine Lake Union Research, University of Washington, Seattle, WA 98109, USA

Received November 15, 2005; Revised and Accepted December 12, 2005

^*To whom correspondence should be addressed at UW Medicine Lake Union, Box 358050, University of Washington, Seattle, WA 98109, USA. Tel: +1 206 616 7949; Fax: +1 206 616 8591; Email: [email protected]

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Nucleic Acids Research 34(1):p e2, 2006.

Chromatin immunoprecipitation (ChIP) is a widely used method to explore in vivo interactions between proteins and DNA. The ChIP assay takes several days to complete, involves several tube transfers and uses either phenol–chlorophorm or spin columns to purify DNA. The traditional ChIP method becomes a challenge when handling multiple samples. We have developed an efficient and rapid Chelex resin-based ChIP procedure that dramatically reduces time of the assay and uses only a single tube to isolate PCR-ready DNA. This method greatly facilitates the probing of chromatin changes over many time points with several antibodies in one experiment.