Stimulation of node and lateral shoot formation in micropropagation of olive (Olea europaea L.) by using dikegulac

Mendoza-de Gyves, Emilio
Rosana Mira, Farida
Ruiu, Fabrizio
Rugini, Eddo

¹Dipartimento Produzione Vegetale, Università degli Studi della Tuscia, Via S. C. de Lellis, 1, Viterbo, 01100, Italy
²Biotech Center, Agency for The Assessment and Application of Technology, Kawasan Puspiptek 630 Bld, Serpong, Indonesia

Received: 26 June 2007; Accepted: 1 November 2007

Plant Cell, Tissue & Organ Culture 92(2):p 233-238, February 2008.

Dikegulac, a growth regulator, was shown to stimulate invitro shoot multiplication of olive cultivars Canino, Frantoio, Moraiolo, but not cultivars Rosciola and Piantone di Moiano. The Rugini Olive Medium including zeatin (4.5μM) was used supplemented with a range of dikegulac concentrations (0–133.4μM). An optimal result in number of shoots and nodes was obtained on cultivars Canino, Frantoio and Moraiolo at 66.7μM dikegulac. Higher concentrations did not stimulate additional shoot and node formation and resulted in a drastic reduction in height of shoots. Elongated shoots were rooted and acclimatised and showed normal development compared to control plants.