The synergistic effect of FC gamma receptor IIa and interleukin-10 genes on the risk to develop systemic lupus erythematosus in Thai population

Hirankarn, N.
Wongpiyabovorn, J.
Hanvivatvong, O.
Netsawang, J.
Akkasilpa, S.
Wongchinsri, J.
Hanvivadhanakul, P.
Korkit, W.
Avihingsanon, Y.

¹Lupus Research Unit, Chulalongkorn University, Bangkok, 10330, Thailand
²Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand
³Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand
⁴Department of Medicine, Nopparat Rajathanee Hospital, Bangkok, 10210, Thailand
⁵Department of Medicine, Faculty of Medicine, Thammasart University, Pathumthani, 12120, Thailand

Correspondence Nattiya Hirankarn, MD, PhD Lupus Research Unit Department of Microbiology Faculty of Medicine Chulalongkorn University, Rama 4 Road Bangkok 10330 Thailand Tel: 662 256-4000 ext 3667 Fax: 662252-5952 e-mail: [email protected]

Received 28 April 2006; revised 17 June 2006, re-revised 25 July 2006; accepted 14 August 2006

Tissue Antigens 68(5):p 399-406, November 2006.

Several linkage analyses have consistently shown that systemic lupus erythematosus (SLE) susceptible genes are located on chromosome 1q21-44. In this study, two major candidate genes, interleukin-10 (IL-10) and Fc gamma receptor IIa (FcγRIIa), within these regions were investigated in Thai SLE patients. The genotyping of three single-nucleotide polymorphisms (promoter area: −1082, −819 and −592) within IL-10 gene and one polymorphism (change amino acid at position 131) within FcγRIIa gene was determined in 195 SLE patients and 159 ethnically matched controls. The RR/RH genotypes of FcγRIIa were found to be significantly increased in SLE patients compared with healthy controls [OR = 2.01, 95% confidence interval (CI) = 1.28–3.14, P= 0.001]. Interestingly, the synergistic effect between RR/RH genotypes of FcγRIIa and ACC/ACC haplotype of IL-10 in susceptibility to SLE was observed (OR = 7.84, 95% CI = 1.60–52.04, P= 0.002). In addition, the FcγRIIa, RR homozygotes was also strongly associated with anticardiolipin antibody production (OR = 6.09, 95% CI = 1.38–30.54, P= 0.006). The result demonstrated that ACC haplotype of IL-10 gene and FcγRIIa R131 polymorphism can be used as marker for genetic susceptibility and severity to SLE in Thai population, particularly individuals carrying both specific genotypes.