A Real-Time Near-Infrared Fluorescence Imaging Method for the Detection of Oral Cancers in Mice Using an Indocyanine Green-Labeled Podoplanin Antibody

  • Ito, Akihiro BA
  • Ohta, Mitsuhiko DDS
  • Kato, Yukinari MD, PhD
  • Inada, Shunko PhD
  • Kato, Toshio MD
  • Nakata, Susumu MD, PhD
  • Yatabe, Yasushi MD, PhD
  • Goto, Mitsuo DDS
  • Kaneda, Norio PhD
  • Kurita, Kenichi DDS
  • Nakanishi, Hayao MD, PhD
  • Yoshida, Kenji DDS

  • 1Graduate School of Pharmaceutical Sciences, Meijo University, Nagoya, Japan

    2Department of Pathology and Molecular Diagnostics, Aichi Cancer Center Central Hospital, Nagoya, Japan

    3The First Department of Oral and Maxillofacial Surgery, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan

    4Department of Oral and Maxillofacial Surgery, Daiyukai General Hospital, Ichinomiya, Japan

    5Department of Regional Innovation, Tohoku University Graduate School of Medicine, Sendai, Japan

    6Information and Communications Headquarters/Graduate School of Information Science, Nagoya University, Nagoya, Japan

    7Department of Diagnostic Pathology, Daiyukai General Hospital, Ichinomiya, Japan

    8Department of Clinical Oncology, Kyoto Pharmaceutical University, Kyoto, Japan

    9Laboratory of Pathology and Clinical Research, Aichi Cancer Center Aichi Hospital, Okazaki, Japan

Hayao Nakanishi, MD, PhD, Laboratory of Pathology and Clinical Research, Aichi Cancer Center Aichi Hospital, Kuriyado, Okazaki, Aichi 444-0011, Japan. Email: e-mail; [email protected]

Received September 24, 2017; revision received December 22, 2017; Accepted February 16, 2018; previously published online April 12, 2018

Technology in Cancer Research and Treatment 17:p 1533033818767936, 2018. | DOI: 10.1177/1533033818767936

Podoplanin is distinctively overexpressed in oral squamous cell carcinoma than oral benign neoplasms and plays a crucial role in the pathogenesis and metastasis of oral squamous cell carcinoma but its diagnostic application is quite limited. Here, we report a new near-infrared fluorescence imaging method using an indocyanine green (ICG)-labeled anti-podoplanin antibody and a desktop/a handheld ICG detection device for the visualization of oral squamous cell carcinoma-xenografted tumors in nude mice. Both near-infrared imaging methods using a desktop (in vivo imaging system: IVIS) and a handheld device (photodynamic eye: PDE) successfully detected oral squamous cell carcinoma tumors in nude mice in a podoplanin expression-dependent manner with comparable sensitivity. Of these 2 devices, only near-infrared imaging methods using a handheld device visualized oral squamous cell carcinoma xenografts in mice in real time. Furthermore, near-infrared imaging methods using the handheld device (PDE) could detect smaller podoplanin-positive oral squamous cell carcinoma tumors than a non-near-infrared, autofluorescence-based imaging method. Based on these results, a near-infrared imaging method using an ICG-labeled anti-podoplanin antibody and a handheld detection device (PDE) allows the sensitive, semiquantitative, and real-time imaging of oral squamous cell carcinoma tumors and therefore represents a useful tool for the detection and subsequent monitoring of malignant oral neoplasms in both preclinical and some clinical settings.

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