Goat Milk Oligosaccharides Are Anti-Inflammatory in Rats with Hapten-Induced Colitis1

  • Daddaoua, Abdelali
  • Puerta, Victor
  • Requena, Pilar
  • Martínez-Férez, Antonio
  • Guadix, Emilia
  • de Medina, Fermín Sanchez
  • Zarzuelo, Antonio
  • Suárez, Maria Dolores
  • Boza, Julio José
  • Martínez-Augustin, Olga

  • *Departments of Biochemistry and Molecular Biology II, **Pharmacology and †Chemical Engineering, University of Granada, Spain and ‡Puleva Biotech S.A., Granada, Spain

1This study was supported by grants (AGL2002-03699, SAF2002-02592, and PPQ2002-02235) from the Spanish Ministry of Science and Technology and by Puleva Biotech, S. A. Olga Martínez-Augustin was funded by the Ramon y Cajal program and Victor Puerta by the Junta de Andalucía.

2To whom correspondence should be addressed. E-mail: [email protected].

Manuscript received 13 October 2005. Initial review completed 1 November 2005. Revision accepted 20 December 2005.

The Journal of Nutrition 136(3):p 672-676, March 2006.

Oligosaccharides are included among the anti-inflammatory components of milk because of their prebiotic properties and their capacity to act as receptors of microorganisms. Here the intestinal anti-inflammatory effect of goat milk oligosaccharides (O) was assessed in trinitrobenzenesulfonic (T) acid-induced colitis in rats. Rats were randomly assigned to three different groups. Two groups (T and OS) of colitic rats and a control group (C) were studied. Group OS received 500 mg/(kg·d) of goat milk oligosaccharides orally, starting 2 d before the colitis induction until d 6, and groups T and C received the vehicle. When compared with the T group, the OS group showed decreased anorexia and body weight loss; reduced bowel wall thickening and longitudinal extension of necrotic lesions; downregulated colonic expression of interleukin 1β, inducible nitric oxide synthase, cyclooxygenase 2, and mucin 3; and increased trefoil factor 3. Thus, goat milk oligosaccharides have anti-inflammatory effects in rats with experimental colitis and may be useful in the management of inflammatory bowel disease. J. Nutr. 136: 672-676, 2006.

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