Comparison of DiaSorin and Bio-Rad Test Kits for the Detection of Hepatitis B Virus Total Core and Surface Antibodies on the Bio-Rad Evolis

Cavalieri, Stephen J. PhD
Hrabovsky, Sharon
Jorgensen, Trichel

Department of Pathology, Creighton University Medical Center, Omaha, NE

Address reprint requests to Dr Cavalieri: Department of Pathology, Creighton University Medical Center, 601 N 30th St, Omaha, NE 68131.

American Journal of Clinical Pathology 133(1):p 110-113, January 01, 2010. | DOI: 10.1309/AJCPHY8P2LOIKFBE

The new MONOLISA Bio-Rad kits were compared to DiaSorin kits for the detection of hepatitis B virus (HBV) total core (HBcTAb) and surface (HBsAb) antibodies on the Bio-Rad Evolis immunoanalyzer. The resolved sensitivities, specificities, positive and negative predictive values, and overall accuracy were 91%, 100%, 100%, 96%, and 97%, respectively, for HBcTAb and 99%, 95%, 96%, 99%, and 97%, respectively, for HBsAb. Whereas accuracy and reagent cost were comparable between the kits, Bio-Rad kits required less specimen volume and less instrument processing time to results than the DiaSorin kits.