SymbolCombination of Schwann cells, small intestinal submucosa and growth factor sustained-release microspheres for repair of peripheral nerve defects
- Zhang, Kai-wei
- Duan, Hong
- Xiang, Zhou
- Xiao, Rui
- Chen, Jiu-yi
1 Department of Orthopedic Surgery, First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550001, Guizhou Province, China
2 Department of Orthopedics, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
3 Department of Orthopedics, First People's Hospital of Yibin, Yibin 644000, Sichuan Province, China
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Zhang Kai-wei☆, Doctor, Associate chief physician, Department of Orthopedic Surgery, First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550001, Guizhou Province, China [email protected]
Corresponding author: Duan Hong, Doctor, Associate chief physician, Department of Orthopedics, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China [email protected]
Supported by: the Key Scientific Plan of Yibin City, No. 200803010*
Received: 2012-07-02
Accepted: 2012-10-19
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Abstract
BACKGROUND:
To combine Schwann cells (SCs) and small intestinal submucosa (SIS) with basic fibroblast growth factor (bFGF) is a feasible way to construct artificial nerve that possesses nerve activity and chemotaxis in vitro.
OBJECTIVE:
To observe the recanalization of neural pathways after repairing peripheral defects with bFGF controlled release microspheres combined with SCs and SIS.
METHODS:
Models of sciatic nerve defects were established in Sprague-Dawley rats, and then randomized into: experimental group repaired with bFGF controlled release microspheres combined with SCs and SIS, positive control group with SCs and SIS combined with free bFGF, negative control group with SCs and SIS, and blank control group with autologous nerve.
RESULTS AND CONCLUSION:
The number of regenerated nerve fibers, number of DiI tracer-positive neurons, positive expression rate of S-100 and neurofilament protein, ultrastructure of regenerated myelin and axons, nerve conduction velocity and composite action potential in the experimental group were superior to those in the positive and negative control groups at 16 weeks after operation (P < 0.05). These findings indicate that bFGF controlled release microspheres combined with SCs and SIS can reconstruct the neural pathways following sciatic nerve injury.
Zhang KW, Duan H, Xiang Z, Xiao R, Chen JY. Combination of Schwann cells, small intestinal submucosa and growth factor sustained-release microspheres for repair of peripheral nerve defects. Zhongguo Zuzhi Gongcheng Yanjiu. 2013;17(3): 457-464.
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