Thyrotropin Receptors in Brown Adipose Tissue: Thyrotropin Stimulates Type II Iodothyronine Deiodinase and Uncoupling Protein-1 in Brown Adipocytes*

Murakami, Masami
Kamiya, Yuji
Morimura, Tadashi
Araki, Osamu
Imamura, Makoto
Ogiwara, Takayuki
Mizuma, Haruo
Mori, Masatomo

First Department of Internal Medicine, Gunma University School of Medicine, Maebashi 371-8511, Japan

Received August 9, 2000.

Address all correspondence and requests for reprints to: Masami Murakami, M.D., First Department of Internal Medicine, Gunma University School of Medicine, Maebashi 371-8511, Japan. E-mail: [email protected].

* This work was supported in part by Grant-in-Aid 09671024 for Scientific Research (to M.Mu.) from the Ministry of Education, Science, and Culture, Japan.

Endocrinology 142(3):p 1195-1201, March 2001.

It has been demonstrated that TSH receptors are expressed not only in thyroid gland but also in extrathyroidal tissues. Brown adipose tissue of guinea pig has been reported to express TSH receptor messenger RNA (mRNA), but the physiological roles of TSH receptors in brown adipose tissue have not been understood. We studied the expression and function of TSH receptors in rat brown adipose tissue and cultured rat brown adipocytes. Northern analysis demonstrated the expression of TSH receptor mRNA in rat brown adipose tissue and cultured rat brown adipocytes. TSH receptor mRNA in rat brown adipose tissue was decreased by cold exposure of the rat, and its mRNA in cultured rat brown adipocytes was also decreased by incubation with TSH or (Bu)₂cAMP. TSH increased the intracellular cAMP concentration in cultured rat brown adipocytes in a dosedependent manner. Type II iodothyronine deiodinase mRNA, its activity, and uncoupling protein-1 mRNA in cultured rat brown adipocytes were significantly increased by incubation with TSH in a dose-dependent manner. These results suggest the expression of functional TSH receptors in brown adipose tissue, which may be involved in regulation of the expression of type II iodothyronine deiodinase and uncoupling protein-1.