A GlcNAc/GalNAc-specific Lectin from the Ascidian Didemnum ternatanum with Effects on the Scacharomyces cerevisiae in Ethanol Fermentation Process

Previously we isolated GlcNAc/GalNAc-specific lectin (DTL-A) from the ascidian Didemnum ternatanum. The purified DTL-A was digested with trypsin and analyzed with nanoESI-MS/MS. Five peptide sequences (LIDTNTGKP, YLDTNTGKI, KLNDLE DALQQAKE, GEMCEEPILSGI and VGMGSILMDHCDVG) were obtained and subjected to the UniProt Knowledgebase (European Bioinformatics Institute) using the WU-BLAST2 network service in a search for proteins that atched the amino acid sequences of DTL-A, but no sequences were retrieved. In studies of its influence on fermentation, DTL-A reveals to be a stimulator for Saccharomyces cerevisiae ethanol production. Ethanol production increased from 1.24 to 1.71% (v/v), when DTL-A concentration in fermentation broth was increased from 0 to 0.0213 mg/L at 12 h. DTL-A shows potent effect on ethanol production from glucose by the S. cerevisiae. Supported by the Natural Science Foundation of China (31071612) and Natural Science Foundation of China (21075012).