qPrimerDepot: a primer database for quantitative real time PCR

Cui, Wenwu
Taub, Dennis D.
Gardner, Kevin

Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, Bethesda, MD 20892-4605, USA
¹Laboratory of Immunology, National Institute on Aging, Baltimore, MD 21224, USA

Received June 3, 2006; Revised September 25, 2006; Accepted September 29, 2006

^*To whom correspondence should be addressed at National Institutes of Health, Advanced Technology Center, Room 134C, 8717 Grovemont Circle, Bethesda, MD 20892-4605, USA. Tel: +1 301 496 1055; Fax: +1 301 435 7558; Email: [email protected]

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Nucleic Acids Research 35:p D805-D809, January 1, 2007.

Gene expression studies employing high throughput real time PCR methods require finding uniform conditions for optimal amplification of multiple targets, often a daunting task. We developed a primer database, qPrimerDepot, which provides optimized primers for all human and mouse RefSeq genes. These primers are designed to amplify desired templates under unified annealing temperature. For most intron-bearing genes, primers flank one of the largest introns thus minimizing background noise due to genomic DNA contamination. The qPrimerDepot database can be accessed at http://primerdepot.nci.nih.gov/ and http://mouseprimerdepot.nci.nih.gov/.