1Mouse Biology Unit, European Molecular Biology Laboratory (EMBL), Via Ramarini 32, 00015 Monterotondo, Italy. 2Pasteur Institute, Foundation Cenci Bolognetti, and Department of Human Physiology and Pharmacology, Center of Excellence BEMM, University of Rome, La Sapienza, Piazzale Aldo Moro 5, 00185 Roma, Italy. 3Department of Anatomy, Pharmacology, and Forensic Medicine, University of Turin, Italy. 4National Institute of Neuroscience, 10126 Turin, Italy.
*To whom correspondence should be addressed. E-mail: [email protected]
Acknowledgments: We thank R. Mellwig, U. Haselmann, and C. Antony at the EMBL Electron Microscopy Core Facility; F. Zonfrillo for expert mouse husbandry; the EMBL Transgenic Faci/ity for help in rederiving mouse lines; J. Griesbach and M. Al Banchaabouchi for help in videoscoring; W. Fouquet at Leica Microsystems, Mannheim, Germany, for access to the STED microscope and microscopy expertise; and A. Pinto, F. Peri, P. Heppenstall and M. Jechlinger for critical discussions. This work was supported by funds from EMBL to C.T.G and R.C.P. and Foundation Cenci Bolognetti to F.P., LM., M.S., and D.R. R.C.P. carried out and analyzed all fluorescent imaging experiments except a portion of the microglia morphological analyses that were carried out by T.AF., L.D., and E.G. Electron microscopy was carried out and analyzed by G.B., P.P., and M.G. Electrophysiological experiments were designed, carried out, and analyzed by M.S., F.P., L.M., and D.R. C.T.G conceived the projects and wrote the manuscript with input from R.C.P. and D.R.
5 January 2011; accepted 13 July 2011
Published online 21 July 2011;
10.1126/science.1202529
